绵羊胚胎发育中后期骨骼肌BTG2/3的表达动态及其与Myostatin的关系

doi:10.11843/j.issn.0366-6964.2015.11.002

Abstract

Abstract:

The aim of this study was to analyze the expression of the sheep BTG2/3 genes in the mid to late embryonic skeletal muscle and to investigate the relationship between MSTN and BTG2/3.Longissimus dorsi of 85 dpc(day postconception)，100 dpc，120 dpc and 135 dpc in Texel and Ujimqin sheep were collected as tested subjects.Real-time PCR was used to detect the expression of MSTN and BTG2/3 in the longissimus dorsi.Semimembranosus，longissimus dorsi，semitendinosus and quadriceps femoris of 100 dpc in the Texel and Ujimqin sheep were selected to detect the expression of BTG2/3.Lentiviral vectors pFU-GW-myostatin were constructed to infect sheep myoblasts at the proliferation and differentiation stage stably and the expression of BTG2/3 were detected at different stages.The results showed that a downward expression trend was emerged of MSTN in Texel fetuses，whereas the expression level in Ujimqin increased after decline at 100 dpc.The expression of BTG2 and BTG3 in Texel had downward trend after rising on 100 dpc，but BTG2 represented decrease-increase-decrease trend and BTG3 represented opposite trend to BTG2 in Ujimqin.Expression levels in different muscle on 100 dpc in the Texel and Ujimqin showed that，BTG2 gene expression was significantly higher(P<0.01) in Texel longissimus dorsi and semitendinosus than that in Ujimqin，and BTG3 gene expression was significantly lower(P<0.01) in Texel semimembranosus and longissimus dorsi than that in Ujimqin.Transfection and interference efficiency of lentiviral vector were very high.After transfected myoblast at proliferation and differentiation stage，respectively，expression levels of BTG2 and BTG3 also were decreased significantly(P<0.01) at both stages.The results indicate that BTG2 and BTG3 play an important role in sheep skeletal muscle growth from the mid to late embryonic stage and may be involved in myostatin regulatory pathway.Our study provide an insight into the molecular regulation mechanism of embryonic development in sheep.

CLC Number:

S826
S813.3

LIU Rui-zao，LIU Zhen，WU Ming-ming，WANG Hui-hua，ZHU Cai-ye，ZHANG Li，ZHAO Fu-ping，WEI Cai-hong，DU Li-xin. Expression Trend of BTG2/3 in the Mid to Late Embryonic Skeletal Muscle of Sheep and the Relationship between BTG2/3 and MSTN[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1916-1923.

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Expression Trend of BTG2/3 in the Mid to Late Embryonic Skeletal Muscle of Sheep and the Relationship between BTG2/3 and MSTN

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